Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNSVIIIESPNKVAKIREITGA---------------------KVFATIGHFMQLKSYDENN------GFKPTFD---YDQEKKKHIFEMIEAC-KNKKVYIATDPDREGYAIGYMFYQKIKNVASSIYRAEFFEITPSGINKGLQNALLFENTNRQMYQSALARRVADMLLGFTLSPYLGKALGQMKGSSAGRVQTPCLKLIVDRDREIEKFKALPENEKVSYQIQAKINDSANREVTIKHCDEKGEEIKFNDKEEALKLFESLKDNKACLLKDLKNSVVETKPKKPFITSTLLEKASSMLGLSISEVQSLAQNLFEAGLITYIRTDAESLSVEFLDETESFYAPIYKDLYLKREYKAGKQSQAEAHEAIRITHPHTTEDLESI-----VYNANITNQDALKLYQLIFERTIESQGKNAIYDKQDLLFKIKNEYFKCSVKGLK----SAGFLAMFSKKELENDESNDDKDNKEKEQNAQFNLKIDDVLSLNDLVLSTIKRNAPSAYKEADFVKLLENKGIGRPSTYASYLPTLVKREYISISQDKKHIITPTHKGKRVVEVFENAYQFIIDLTYTKQMEEVLDEIVENKSSYVDFISNLNSKCPKIEKLERNDDEIKPSSEGQITYIENILRDLQLNLSEEFKNYKEDNRVAKAFLDRYIKEHEFFKKNNKKASSSNNDENRPATPKQISFAEILAKKHNVKLPKGFKYSMKVCGDFINEYHKK 1GKU Chain:B ((505-1053)) -KPALFIVESPTKARQISRFFGKPSVKVLDGAVVYEIPMQKYVLMVTASIGHVVDLITNRGFHGVLVNGRFVPVYASIK--DNSRSRIEALRKLAHDAEFVIVGTDPDTEGEKIAWDLKNLLSG-CGAVKRAEFHEVTRRAILEALESLRD---VDENLVKAQVVRRIEDRWIGFVLSQKLWERFNN-RNLSAGRAQTLVLGWIIDRFQESRERRKIA-----IVR-----------DFDLVL--------EH-DEEE-----------FDLTIKLVEEREELRTPLPPYTTETMLSDANRILKFSVKQTMQIAQELFENGLITYHRTDSTRVSDVGQRIAKEYLGD----DFVGR---E--WGESGAHECIRPTRPLTRDDVQRLIQEGVLVV-EGLRWEHFALYDLIFRRFMASQCRPFKVVVKKYSIEFDGKTAEEERIVRAEGRAYELYRAVWVK-------------------NELP---TGT-FR---VKAEVKSVPKVLPFTQSEIIQMMKERGIGRPSTYATIVDRLFMRNYVVEK---YGRMIPTKLGIDVFRFLVRRYAKFVSEDRTRDLESRMDAIERGELDYLKALEDMYAEIKSID---------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1GKU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -147268 for 3916 contacts (-37.6/contact) + 2D Compatibility (PS) -50423 + (NN) 6145 + (LL) 11536 1D Compatibility (HY) -26400 + (ID) 6050 Total energy: -212460.0 ( -54.25 by residue) QMean score : 0.365

(partial model without unconserved sides chains): PDB file : Tito_1GKU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GKU-query.scw PDB file : Tito_Scwrl_1GKU.pdb: