Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYSTIFNIGQINKYSKLAIFMSILFLCGCSSQTHSSQKETTIPVTLHVEDA-KGLPVEGVQVTIVKAPSSDEEPSTEIGEILGKTDKNGDIKWDT----GRKGDYSVALTKGETSVTHHISLTEDKKDHAIPLVFKE 5AYT Chain:A ((42-111)) -----------------------------------------CPLMVKVLDAVRGSPAINVAVHVFRKAAD----DTWEPFASGKTSESGELHGLTTEEEFVEGIYKVEIDTKSYW----------------------

General information: TITO was launched using: RESULT: Template: 5AYT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 231 -15309 -66.27 -235.52 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -66.27 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.466

(partial model without unconserved sides chains): PDB file : Tito_5AYT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AYT-query.scw PDB file : Tito_Scwrl_5AYT.pdb: