Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIQDSMQFAAVESGLRFYQAYDQSLSLWPIESEAFYVSTRFG-KTHIIASGPKDAPSLILLHGGLFSSAMWYPNIAAWS-SQFRTYAVDIIGDKNKSIPSAAMETRADF-AEWMKDVFDSLGLETAHLAGLSLGGSHIVNFLLRAPERVERAVVISPAEAFISFHPDVYKYAAELTGARGAESYIKWITGDSYDLHPLLQRQIVAGVEWQDEQRSLKPTENGFPYVFTDQELKSIQVPVLLMFGEHEAMYHQQMAFERASVLVPGIQAEIVKNAGHLLSLEQPEYVNQRVLSFLCGGIK 1CR6 Chain:B ((239-367)) -----------------------------------YVTVKPGIRLHFVEMG--SGPALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIEEYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERV-RAVA-SLNTPFMPPDPDV-----------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1CR6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 606 -95660 -157.85 -759.20 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.60 3D Compatibility (PKB) : -157.85 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.426

(partial model without unconserved sides chains): PDB file : Tito_1CR6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1CR6-query.scw PDB file : Tito_Scwrl_1CR6.pdb: