Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQQILEHIVGIANDLLWSKLLIVLLLSFG----------IYFTFRLKFLQVRMLKEMVRVLREGAASRSKNSI--SPFQAFCISMAARVGTGNITGIAIAIALGGPGAIFWMWIIAIIGSASSFVESTLAQIYKVKDVNGFRGGPAYYMEKGLNKRWMGALFAVLITLSFGIVFNSVQSNTVSLAFENAFGTNRLTLGLILIAVFGTIIFGGVKRIAKLAESIVVVLAVLYIGVAFFVIFSNITQLPGVLALIVKNAFGFDQAAGGALGAALMQGVRRGIFSNEAG--MGSAPNAAATATTSHPVKQGLIQAFGVLTDTLVICTSTAFIILFSDAYHTPGLSGIALTQASLSS-----HVGSWASGFLAILILLFGFCALIGNYYYGETNIGFLNKS---KKLIFVYRIGVLAMIVFGCVAKVQLVWDLADLFMGLMVIVNLIAIFLLSKVVFTALKDYTRQKKAGKDPVFYKDVLKNHNGIECWP-VSDTKTDTHNKQIS---------- 4ZFV Chain:A ((10-445)) --NVLDFTVLGLNSGTSMDGIDCALCHFYQKTPDAPMEFELLEYGEVPLAQPIKQRVMRMILEDTTSPSELSEVNVILGEHFADAVRQFAAERNVDLSTIDAIASHGQTIWLLSMP---------------------------------EEGQVKSALTMAEGAILASRTGI-------TSITDFRISDQAAGRQ--GAPLIAFFDALLLHHPTKL-RACQNIGGIANVCFIP-------------PDVDGRRTDEYYDFDTGPGNVFIDAVVRHFTNGEQEYDKDGAMGKRGKVDQELVDDFLKMPYFQLDPPKTTGREVFRDTLAHDLIRRAEAKGLSPDDIVATTTRITAQAIVDHYRRYAPSQEIDEIFMCG------GGAYNPNIVEFIQQSYPNTKIMMLDEAGVP----AGAKEAITFAWQGMEALVGRSIPVP-------TRVETRQHYVLGKVSPGLNYRSVMKKGMAFGGDAQQLPWVSEMIVKKKGKVITNNWAGSHHHH

General information: TITO was launched using: RESULT: Template: 4ZFV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2387 -283116 -118.61 -702.52 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -118.61 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.224

(partial model without unconserved sides chains): PDB file : Tito_4ZFV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZFV-query.scw PDB file : Tito_Scwrl_4ZFV.pdb: