Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------------------------------MAMNLRKNQAPLYIKVHEIDNTAIIVNDGGLPKGTVFSCGLVLEEDVPQGHKVAL---------TDLNQGDEIVRYGEVIGFADE--TIKRGSWIREALVRM---PAPPALDDLP-LANRVPQPRPPLEGYTFEGYRNADGSAGTKNILGITTSVQCVVGVLDY-----AVKRIKEELLPKYPNVDDVVPLHHQYGCGVAINAPDAVIPIRTIQNLAKHPNFGGEVMVIGL--------GCEKLLPERIA--SENDDDILSLQDHRGFAAMIQSI--LEMAEERLIRLNSR-----TRVSCPVSDLVIGLQCGGSDAFSGVTANPAVGYAADLLVRAGATV---LFSEVTEV-RDAIHLLTP-RAVSEEVGQSLIKEMKWYDSYLRRGDADRSANPSPGNKKGGLSNVVEKALGSVAKSGTSPISGVLGPGERAKQKGLLFAATPASDFVCGTLQLAAGMNLQVFTTGRGTPYGLAAAPVLKVSTRHSLSEHWADLIDINAGRIATGEASIEDVGWEIFRTILDVASGRKQTWADRWGLHNDLCLFNPAPVT-- 4UZY Chain:A ((9-650)) QQPARPIAEGQYTQTIYTLIKEQKFAEAIQHLQYQLQNVPESRAALSLLGYCYYYTGQYDMASQMYEQLVTLYPSNEDYKLYYAQSLYKGGMYPEASKAVVKVEGHQKAVTTLLVACSYEQDDLTGCRRQLDKCAPEDPDTMVNTGCIMFKEGKFEAARQKFNDYQPELLYNIALCYYKTKQFGPALKHLAEIIEKAVREHPELSVGSDGMEVRSVGNSQTLKETALIEAFNLKAAIEYTMKNVEAAKEALTDMPPRAEEELDPVTLHNSALIN---MDSDPTGGFKKLNFLLQSPPFPPETFANLLLLYCKPSHGFYDLAADVLAENPQYAGKLLSPDLYDYLQAAIGRYKSPEEAFRRFDELATRHVEQLRRLTKQIQDARIARDNDAIKRAINEYDEALEAYIPGLMAMASIYWDMELYSNVEKIFRQSAEFCSEHEVWKLNVAHTFFMQDNHYKEAIRYYEPVVKKNAD--NLLGVTAIVLANLCVSYIMTSQNEEAEELMRKVEKEEERSSMQDPDKPCFHLCIINLVIG-TLYCAKGNYEFGVSRIIKSLEETDTWYYAKRCFLALIENLAKHMIVLKDSSFTEIMAFLNEAEKHGKDIRVVFNQSRTIASEARMLKKMFLKLR

General information: TITO was launched using: RESULT: Template: 4UZY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1985 -157448 -79.32 -312.40 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -79.32 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.145

(partial model without unconserved sides chains): PDB file : Tito_4UZY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UZY-query.scw PDB file : Tito_Scwrl_4UZY.pdb: