Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITLFQCLYLILFSFICYQGAAAFSHSTAASWLAAALGAAAAGLYIWNTKRVWKHCSSGLCAWIAVIQVMSVGVVLIGTDIMPVLCVIAIFAGCEGLRIGQSALQARLSDQIDKLTQAEQHANQMLIDVRSRNHDTMKHITAIKSA-----Q---P-------KADTQAYIQNWADQ--YSQYDRFLKG-----------------------ENAYVAGVLYDFLEKARASN--------VSVSLHMHTPLSSLPFSPADQVSLVGNILENALDSAAEARE----KAEIKLETSLRSGLYVLTCENSTPGMDPKVLDTIYQSFGRSTK-----NGAHEGMGTYIIQKLVKGAFGRLDFTYRHP-IFRLEIKIPFQK 2E0A Chain:A ((94-350)) --------------------------------------------------------------------------------------------------------------------------KALSDFVDTLIKVRNRHHNVVPTMAQGIIEYKDACTVDPVTNQNLQYFLDRFYMNRISTRML-MNQHILIFSDSQTGNPSHIGSIDPNCDVVAVVQDAFECSRMLCDQYYLSSPELKLTQVNGKFPDQPIHIVYVPSHLHHMLFELFKNAMRATVEHQENQPSLTPIEVIVVLGKEDLTIKISDRGGGVPLRIIDRLFS-YTYSTAPTPNAPLAGFGYGLPISRLYAKYFQGDLNLYSLSGYGTDAIIYLKAL-

General information: TITO was launched using: RESULT: Template: 2E0A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 787 -86746 -110.22 -449.46 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -110.22 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.402

(partial model without unconserved sides chains): PDB file : Tito_2E0A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2E0A-query.scw PDB file : Tito_Scwrl_2E0A.pdb: