Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLMNEFEKACETLRKFMAYMLEK-DMKSWTELWDENAVFEFPYAPEGSPKRIEGKAAIYDYIKDYPKQIHLSSFTAPTVYRSADSNTVIAEFQCDGHVIETGLPYRQSYISVIETRDGRIVRYRDYWNPLVVKEAFGGSFLQTEESGK 4J8T Chain:D ((1-128)) --MNAKEIVVHALR-----LLENGDARGWCDLFHPEGVLEYPYPPPGYKTRFEGRETIWAHMRLFPEYMTIR-FTDVQFYETADPDLAIGEFHGDGVH--SGGKLAADYISVLRTRDGQILLYRLFFNPLRVLEPL------------

General information: TITO was launched using: RESULT: Template: 4J8T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 548 -60401 -110.22 -483.21 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain D : 0.76 3D Compatibility (PKB) : -110.22 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.592

(partial model without unconserved sides chains): PDB file : Tito_4J8T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J8T-query.scw PDB file : Tito_Scwrl_4J8T.pdb: