Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKPKKRQMEYLTRGLIAVQTEQGVFVSWRFLGTDHETTAFHLYRDGKRITRDPIAESTNFLDQNGTADSVYQVAAVNKGREEKLSKKARVWQENVLEVPLAKPEGGVTPDGKPYTYSANDASVGDIDGDGEYEMILKWDPSNSKDNAHDGYTGEVLIDAYKLDGTFLWRINLGRNIRAGAHYTQFMVYDLDGDGKAEIAMKTADGTTDGKGHIIGDEQADFRNEQGRILSGPEYLTVFKGETGEALTTVEYEPPRGKLEDWGDG-YGNRMDRFLAGTAYLDGERPSLVMARGYYTRTVLVAYDFRNGRLKKRWVFDSNQPGHEAYAGQGNHSLSVADVDGDGKDEIIY-------GAMAVDHDGTGLYSTGLGHGDAMHVGDLDPSRKGLEVFQVHEDATKPYGLSLRDAGTGEILWGVHAGTDVGRGMAAHIDPSYKGSLVWGIDPPGNDGMSYGLFTSKGEKISDK----APSSANFAIWWD---GDLVRELLDHDWDGTIGR----PKIEKW---DAENGCLKTIFQPAGVLSNNGTKGNPVLQANLFGDWREEVIWRTEDSSALRIYTTTHLTRHCFYTLMHDPVYRLGIAWQNTAYNQPPHTSFYLGTGMKKPPKPALYIAGSKAEAPL----------------------- 5CVL Chain:A ((27-578)) --RRKVQVSYVIRDEVEKYNRNGVNA----LQLDPAL--NRLFTAGR--------------------DSIIRIWSVNQHKQDPYIASMEHHTDWVNDIVLCC-------NGKTLISASSDTTVKVWN-------------------AHKGFCMSTL--------------------RTHKDYVKALAYAKD---KELVASAGLDRQ----------------------------IFLWDVNTLTALTASNNTVTTSSLSGNKDSIYSLAMNQL--GTIIVSGSTEKVLRVWDPRTCAKLMKLKGHTDNVKALLL---NRDGTQCLSGSSDGTIRLWSL-GQQRCIATYRVHDEGVWALQVNDAFTHVYSGG----RDRKIYCTDLRNPDIRVLICEEKAPVLKMELDRSADPPPAIWVATTKSTVNKWTLKGIHNFRASGPITPLCTQPDQVIKGGASIIQCHILNDKRHILTKDTNNNVAYWDVLKACKVEDLGKVDFEDEIKKRFKMVYVPNWFSVDLKTGMLTITLDESDCFA-------AWVSAKDAG-------FSSPDGSDPKLNLGGLLLQALLEYWPRTHVNPMDKG--NGYFQVPPHTPVIFGEAGGRTLFRLLCRDSGGETESMLLNETVPQWVIDITVDKNMPKFN

General information: TITO was launched using: RESULT: Template: 5CVL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2783 -122381 -43.97 -254.43 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -43.97 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.206

(partial model without unconserved sides chains): PDB file : Tito_5CVL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5CVL-query.scw PDB file : Tito_Scwrl_5CVL.pdb: