Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEALTEQKSQKWAISLFTIGVFMAALDNGIISAALTTINESFS--------------VSPSWGSWGITLYTLGLSVSVPIVGKLSDRYGRKKLFLIEVCLFGLGSLLVAL---SQSFPLFLISRLIQALGGGGIFIIGSSHILATLPKEKQGKALGLLGAMNGMAAVLGPNIGSFLLD-WTGSWHWLFLINLPIAVLLVVFGACFIAETKAPEAKRLDAAGIFLLSLSILAVMYGMTNLDGANLLHSLGNPEVYGCIIFGILCFAALISYEKRVEMRGGDPILAYSLLRNHMFQRTLIIGLLSG-G-----LLAAVIFIPSYVEQYLGVPAAKAGYWMTPLALASGIGAWLGGALTDKKGPVKTVILSGIISCAGFALFPLWV------T--EKWEFVIASVAAGIGFGFLLGAPLNVLVSEAAK-TNKGTALGTLSLVRQIGLTLAPTLYAGFITAGFDQIGDEINSRLSDSGYSEKAMQMIPEIDSSEVSSLQEQIERIPVPEVKTAISDAIHASVASGYDHLYAAAAVVSLLVIAAISIPAFRRQKR 4ZW9 Chain:A ((40-447)) ------------------TIGSFQFGYNTGVINAPEKIIKEFITKTLTDKGNAPPSEVLLTSLWSLSVAIFSVGGMIGSFSVGLFVNRFGRRNSMLIVNLLAVTGGCFMGLCKVAKSVEMLILGRLVIGLFCGLCTGFVPMYIGEISPTALRGAFGTLNQLGIVVGILVAQIFGLEFILGSEELWPLLLGFTILPAILQSA-ALPFCPESPRFLLINRKEEENAKQI---LQRLWGTQDV----------SQDIQ-------------EMKDESARMSQEKQVTVLELFRVSSYRQPIIISIVLQLSQQLSGINAVFYYSTGIFKD---AGVQEPIYATIGAGVVNTIFTVVSLFLVERAGRRTLHMIGLGGMAFCSTLMTVSLLLKDNYNGMSFVCIGAILVFVAFFEIGPG-PIPWFIVAELFSQGPRPAAMAVAGCSNWTSNFLVGLLFPSAAH----------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4ZW9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1897 -230072 -121.28 -613.52 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -121.28 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.216

(partial model without unconserved sides chains): PDB file : Tito_4ZW9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZW9-query.scw PDB file : Tito_Scwrl_4ZW9.pdb: