Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIVALHVVLYQPEIPANTGNIARTCAATNTTLHLIRPLGFSTDDKMLKRAGLDYWEFVNVVYHDSLEELF--EAYKKGKFFFITKFGQQPHTSFDYTDLDEDYFFVFGRETSGLPKDLIQN-NMDRCLRLPMTEHVRSLNLSNTAAILVYEALRQQNYRDLK 4KGN Chain:F ((6-156)) -----FNVVLVEPEIPPNTGNVIRLCANTGARLHLIEPLGFPLDDAKMRRAGLDYHEYAQMRVHRDWDAFVAAEAPDPARMFAFTTRGS---GRFHDRAFEPGDWFVFGAETRGLAPALVDRFAPEQRVRLPMRPGNRSLNLSNTVAVVVFEAWRQAGF----

General information: TITO was launched using: RESULT: Template: 4KGN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 647 -102726 -158.77 -694.09 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain F : 0.81 3D Compatibility (PKB) : -158.77 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.556

(partial model without unconserved sides chains): PDB file : Tito_4KGN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4KGN-query.scw PDB file : Tito_Scwrl_4KGN.pdb: