Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDSMDHRIERL-EYYIQLLVKTVDMDRYPFY--ALLIDKGLSKEEGEAVMRICD--ELSEELATQKAQGFVT-----FDKLLALFAGQLNEKLDVHET----------IFALYEQGLYQELM-----------EVFIDIMKHFD--- 3FHK Chain:A ((1-147)) SNAMSMAYEEYMRQLVVPMRRELTGAGFEELTTAEEVENFMEKAEGTTLVVVNSVCGCAAGLARPAATQAVLQNDKTPDNTVTVFAGQDKEATAKMREYFTGAAPSSPSMALLKGKEVVHFIPRHEIEGHDMEEIMKNLTAAFDAHC

General information: TITO was launched using: RESULT: Template: 3FHK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 450 -62737 -139.41 -555.19 target 2D structure prediction score : 0.30 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -139.41 2D Compatibility (Sec. Struct. Predict.) : 0.30 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.323

(partial model without unconserved sides chains): PDB file : Tito_3FHK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FHK-query.scw PDB file : Tito_Scwrl_3FHK.pdb: