Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDNDKFKEELAKLPEVDPMTKMLVQNIFSKHGVTKDKMKKVSDEEKEMLLNL------VKDLQAKSQALIENQKKKKEEAAAQ-------------------------EQKNTKPLSRREQLIEQIRQRRKNDNN----------------- 2ECU Chain:A ((1-149)) --MKEAFKEALARFASG--VTVVAARLGEEERGMTATAFMSLSLEPPLVALAVSERAKLLPVLEGAGAFTVSLLREGQEAVSEHFAGRPKEGIALEEGRVKGALAVLRCRLHALYPGGDHRIVVGLVEEVELGEGGPPLVYFQRGYRRLVWPS

General information: TITO was launched using: RESULT: Template: 2ECU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 347 -22344 -64.39 -221.23 target 2D structure prediction score : 0.29 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -64.39 2D Compatibility (Sec. Struct. Predict.) : 0.29 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.186

(partial model without unconserved sides chains): PDB file : Tito_2ECU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ECU-query.scw PDB file : Tito_Scwrl_2ECU.pdb: