Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKYGIVLFPS-KKLQDLANSYRKRYD-----PSYSLIPP---HLTLRASFECA-------------EEKADQLVSHLRNIAKE---SHPLVLKMTKYSSFAPVNNVIYIKAEP--TEELKTLNEKLYTGVLAG-EQEYNFVPHVTVGQNLSDDE---HS---DVLGQLKMQ--EVSHEEIVDRFHLLYQLENGSWTVYETFLLGRGE 2FSQ Chain:A ((44-227)) --TVVCHLVEGSQTESAIVSTRQRFLDMPEASQLAFTPVSSLHMTVFQGVIESRRALPYWPQTLPLDTPIDAVTDYYRDRLSTFPTLPAFNMRVTGLRPVG----MVMKGATAEDDSIVALWRDTFADFFGYRHPDHDTYEFHITLSYIVSWFEPECLPRWQAMLDEELEKLRVAAPVIQMRPPAFCEFK-----------------

General information: TITO was launched using: RESULT: Template: 2FSQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 534 -80057 -149.92 -540.92 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -149.92 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.475

(partial model without unconserved sides chains): PDB file : Tito_2FSQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FSQ-query.scw PDB file : Tito_Scwrl_2FSQ.pdb: