Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKKAVIITGGSSGMGKAMAKKQAELGWHVMVTGRNHEALEETKKEIQ-----TFEGQVACFQMDVRSDSAASDMIKEAVKAFGRLDALINNAAGNFICPAEKLTPNGWKAVIEIVLNGTFFCSQAAARHWIDQKQQGVILNMAATYAWGAGAGVVHSAAAKAGVLSLTRTLAVEWGSKYGIRTNAIAPGPIERTGGAEKLFESEKAMARTMNSVPLGRLGTPEEIAALAAFLLSDEASYINGDCITMDGGQWLNPYPF 1YXM Chain:B ((16-272)) LQGQVAIVTGGATGIGKAIVKELLELGSNVVIASRKLERLKSAADELQANLPPTKQARVIPIQCNIRNEEEVNNLVKSTLDTFGKINFLVNNGGGQFLSPAEHISSKGWHAVLETNLTGTFYMCKAVYSSWM-KEHGGSIVNIIVPTKAGFPLA-VHSGAARAGVYNLTKSLALEWACS-GIRINCVAPGVIYSQTA-------QSFFEGSFQKIPAKRIGVPEEVSSVVCFLLSPAASFITGQSVDVDGGRSLYTHSY

General information: TITO was launched using: RESULT: Template: 1YXM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1349 -155015 -114.91 -635.31 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : -114.91 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.528

(partial model without unconserved sides chains): PDB file : Tito_1YXM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YXM-query.scw PDB file : Tito_Scwrl_1YXM.pdb: