Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------MKSIGVVRKVDELGRIVMPI-----ELRRALDIAIKD---SIEFFVDGDKIILKKYKPHGVCLMTGEITSENK-EYGNGKITLSPEGAQLLLEEIQAALKE 1A8Y Chain:? ((127-235)) VELIEGERELQAFENIEDEIKLIGYFKNKDSEHYKAFKEAAEEFHPYIPFFATFDSKVAKKLT-----LKLNEIDFYEAFMEEPVTIPDKPNSEEEIVNFVEEHRRS

General information: TITO was launched using: RESULT: Template: 1A8Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 351 -31029 -88.40 -356.65 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -88.40 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.506

(partial model without unconserved sides chains): PDB file : Tito_1A8Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1A8Y-query.scw PDB file : Tito_Scwrl_1A8Y.pdb: