Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIYLDVI---WLLNFCFDALLLLLT------------AFILKRHVKKRRLVGGAFIGSSIVLLMFTPFSPIVEHPAGKLAFSVV------IVVVTFGFKRFRFFFQNLFSFYFATFLM---GGGIIGAHSLLQ---SNSIVQNGVMITNQTGFGDPISWLFIVGGF--PALWFFS------KRRIEDIETKNIQYEERVSVQADL-----GSQTLHVRG------LIDSGNQLYDPLTKTPVMIIYIDKLEPIFGTAETMIIRNTDPLEAIEQLDD-SFRFLDKMRLIPYRGVGQQNQFLLCVKPDHVTIMTKEEMISADKCLIGISTTKLSADGEFDAIIHPKMLSGKAVKHVS------------------------ 1ECX Chain:A ((1-376)) MRVYFDNNATTRVDDRVLEEMIVFYREKYGNPNSAHGMGIEANLHMEKAREKVAKVLGVSPSEIFFTSCATESINWILKTVAETFEKRKRTIITTPIEHKAVLETMKYLSMKGFKVKYVPVDSRGVVKLEELEKLVDEDTFLVSIMAANNEVGTIQPVEDVTRIVKKKNKETLVHVDAVQTIGKIPFSLEKLEV---DYASFSAHKFHGPKGVGITYIRKGVPIRPLIHGGGQERGLRSGTQ-------NVPGIVGAARAMEIAVEELSEAAKHMEKLRSKLVSGLMNLGAHIITPLEISL----PNTLSVSFPN--IRGSTLQNLLSGYGIYVSTHVLDAMGVDRRIAQGAIRISLCKYNTEEEVDYFLKKIEEILSFL

General information: TITO was launched using: RESULT: Template: 1ECX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1434 -169207 -118.00 -577.50 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -118.00 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.249

(partial model without unconserved sides chains): PDB file : Tito_1ECX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ECX-query.scw PDB file : Tito_Scwrl_1ECX.pdb: