Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MKRVLFS-----VIVFTA-------V-GFTFCQSKAHALTFTVLPITQKTD-----------QWSVKVSEAKNVKE------------FTRPH------------KGEYQ---------VYSLEVKNIGEKAATVDVQLYRNDPNSITRFSLFGCPDENCVKPKEDS-KMLAESLNDGSPL-KFNHFMLADKASELEVVIIWTQKGQEGRN--LKQTFKFTEDGVN------------------ 5CW4 Chain:A ((6-251)) SSLQKVELQTDVYMVCLQHALSTENFEVMGLLIGNFACGIAKISAVIILRRLDKKKDRVEISSEQLLKAAAEAERLTVELNRPMRVLGWYHSHPHITVCPSHVDVRTQATYQTMDHSFVGLIFSVFSEGKESKEHEIFLNCFQSDNGEATEIPLEIVHTPDISDRCLRTMTDLSKILVQEEEDMAEACKDHPDVLASIHNNAVRTRALIHITDIITKPLVQTFEKRIALNKLRATHLQRQLQELQK

General information: TITO was launched using: RESULT: Template: 5CW4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 799 32341 40.48 196.01 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 40.48 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.284

(partial model without unconserved sides chains): PDB file : Tito_5CW4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5CW4-query.scw PDB file : Tito_Scwrl_5CW4.pdb: