Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKAIIKEDVQASLERYADRPVYIHLETTTGSYSAHLNEKNMTVVAYIRNAKVTYHQAKIKGNGPYRVGLKTEEGWIYAEGLTEYTVDEENRLLMAGHLPGGKLAISLQISEKPFTV 3KTS Chain:A ((43-67)) -----LKALVKYAQAG-GKKVLLHADLVNGL-------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3KTS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 23 -4494 -195.39 -179.76 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : -195.39 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.615

(partial model without unconserved sides chains): PDB file : Tito_3KTS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3KTS-query.scw PDB file : Tito_Scwrl_3KTS.pdb: