Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRFILVLSFLSIIVAYPIQTNASPMPCSVILEPVDKNLKNAKGVALIYKVQLNPPSAARTNISILAVHLPAPSSFGNYDSYEGFATKPGEISWRFKLYPTPEEESPSWAGRIDT-ISAEMKNVKVQVRLSNSSTQKLGPSILTKNIESCY 2MQ8 Chain:A ((1-112)) -----------MLTVEVEVKITADD-------ENKAEEIVKRVIDEVEREVQKQYPNATITRTLTRD---------------------DGTVELRIKVKADTEEKAKSIIKLIEERIEEELRKRDPNATITRTVRTEVGSSWSLEHHHHHH

General information: TITO was launched using: RESULT: Template: 2MQ8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 464 549 1.18 4.94 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 1.18 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.191

(partial model without unconserved sides chains): PDB file : Tito_2MQ8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MQ8-query.scw PDB file : Tito_Scwrl_2MQ8.pdb: