Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MENIALESSFLEYDINEPIKIYTGHFTIEVADDFFEILGEVKIAFLPKARLIFEGAISGNLSKLFEFEKAMKSNNMMINVPGFMKSEVLISGITDGSKGNKVSGILKRSILTSAETKVNRMEFTVVNFVNDLGRRIVHGRFKFSGR----TKLKYKD-WEIILDKRYDYSNKKIFDRLKNSGGYLITHVGYLKRVDDKLFDTKEVEPLISGLYWLLSFSAGRHVAIPTLEGYHNE--EVIWSKYQVPLIDGWTNNITWFPKQKSPSLEHLFPKVIEKQEDPFWNKVL-------WEVLSWYSQAHSSSIVENKVVSVQVALET-----LAWVYLIVDRKSNISKSKYKYMNAAEKFREILSRFSIDLSIPKLFIDIKDNYDDGPHLFTVFRNKIVHPTRELDFDNPIDKLHVLYLGVWYLELLTLGILGYEGSYVNRLKVPIIEGVYEFVPWKTRDN 4J1Q Chain:A ((20-445)) HMERLMLEPVWEKQ--NEELS-YTEHIIVLFETE--RSVTDSIASHMKDARVITLNEAVGHIAERYQCYMQNIFELLQSKVRKLSAGRIIIQAIVPLEKEKQLFAGVS-GLFKTAEIEFSKLTAQVIEIEKPEEMIDLHLKLKDDSRRPFDKQIRYEAGYRFVKGWREMVDTLHM--PWRDEGVYLIT--GGAGSLG-LLFAKEIANRTGRSTIVLTGRSVLSEDKENELEALRSIGAEVVYREADVS---------------DQHAVRHLLEEIKERYGT--LNGIIHGAGSSKDRFIIHKTNEEFQEVLQPKVSGLLHVDECSKDFPLDFFIFFSSVSGCLGNAGQADYAAANSFMDAFAEYRRSLAASK-----------KRFGSTISFNWPLWEEGGMQVGAEDEKRMLKTTGMVPMPTDSGLKAFYQGIVSDKPQVFVMEG--QLQKMKQKLL

General information: TITO was launched using: RESULT: Template: 4J1Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2187 14021 6.41 35.32 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 6.41 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.264

(partial model without unconserved sides chains): PDB file : Tito_4J1Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J1Q-query.scw PDB file : Tito_Scwrl_4J1Q.pdb: