Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------MKLWMRKTLVVLFTIVTFGLVSPPAALMADKPSGQPSSLEQND--------YTAFYDEHDL----------------YDDESDDRRDPELLFQSYKEQLLD----SAEDQSFLKFGSRIAPVIEDDYRKEILPKIENVISDYLATLQDD---------EAYQDVVISSMPSAGKTEKIFNVYNRTTGEDLLRFHVRRDHPPHDGYWFNFHYHTAEDGFQSHHELGSIYWDRNTPPNWMSA---------------- 2ZIH Chain:A ((62-343)) INIPTLTLMEEVLLMGLRDREGYLSFWNDSISYALRGCIIIELALRGKIRILDDSARKRFDLSERLIEVIDSSKTGEVLLDETLQLMKNDEPLSISNWIDLLSGETWNLLKINYQLKQVRERLAKGLVDKGVLRTEMKNFFLFDMATHPIADASCKEAIKRRVLSVLVSRNMELSYNEYFPETTSFKIIRTLALICGSYG--ANVLENVLTTLEYEKRDKAISRAEEIMAQFSQYPFDLEKETELGVSVNLNKEVKEEIENNPGHDLQLEVIAGVFEVFSRMDM

General information: TITO was launched using: RESULT: Template: 2ZIH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 819 31835 38.87 158.38 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : 38.87 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.229

(partial model without unconserved sides chains): PDB file : Tito_2ZIH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZIH-query.scw PDB file : Tito_Scwrl_2ZIH.pdb: