Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTNRHQALGLTDQEAVDMYRTMLLARKIDERMWLLNRSGKIPFVISCQGQEAAQVGAAFALDREMDYVLPYYRDMGVVLAFGMTAKDLMMSGFAKAADPNSGGRQMPGHFGQKKNRIVTGSSPVTTQVPHAVGIALAGRMEKKDIAAFVTFGEGSSNQGDFHEGANFAAVHKLPVIFMCENNKYAISVPYDKQVACENISDRAIGYGMPGVTVNGNDPLEVYQAVKEARERARRGEGPTLIETISYRLTPHSSDDDDSSYRGREEVEEAKKSDPLLTYQAYLKETGLLSDEIEQTMLDEIMAIVNEATDEAENAPYAAPESALDYVYAK 3DV0 Chain:G ((42-337)) ----------LSDEQLKELMRRMVYTRILDQRSISLNRQGRLGFYAPTAGQEASQIASHFALEKE-DFILPGYRDVPQIIWHGLPLYQAFL--FSRG---HFHGNQIPEGVNVLPPQIIIGA-----QYIQAAGVALGLKMRGKKAVAITYTGDGGTSQGDFYEGINFAGAFKAPAIFVVQNNRFAISTPVEKQTVAKTLAQKAVAAGIPGIQVDGMDPLAVYAAVKAARERAINGEGPTLIETLCFRYGPHT---DPT--------NEWAKKDPLVRFRKFLEAKGLWSEEEENNVIEQAKEEIKEAIKKADETP--------------

General information: TITO was launched using: RESULT: Template: 3DV0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 1607 -108143 -67.29 -380.79 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain G : 0.81 3D Compatibility (PKB) : -67.29 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_3DV0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DV0-query.scw PDB file : Tito_Scwrl_3DV0.pdb: