Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRFSTAYLLL--GILCSAAVFLIGAPSRALGAEVEHYEPLQVHVQLEKVYLDGDVSIEHKHEKVFSMDDFWAAYAGWTLVEQKKGYVLFRKQMDDISPLSKVNGYIGVSDNGVISTFHGRPEPASEPIQ-----SFFQIDLERLESHMQK--NLLKGIPFRTKAEFEDVIEHMKTYSG 1A9X Chain:B ((1-149)) ---IKSALLVLEDGTQFHGRAI--GATGSAVG---------EVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNVGTNDADEE-------------SSQVHAQGLVIRDLPLIASNFRNT-EDLSSYLKRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPG

General information: TITO was launched using: RESULT: Template: 1A9X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 785 19135 24.38 136.68 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : 24.38 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.166

(partial model without unconserved sides chains): PDB file : Tito_1A9X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1A9X-query.scw PDB file : Tito_Scwrl_1A9X.pdb: