Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------MYFVDRSKIEKT--------LGFFEHQLALFDSQT-----DWQSEIGELALQR-------IGHLLIECILDTGNDMIDGFIMRDPGSYDDIMDILVDEKVVTEKEGDELKKLIAYRKTLVQQY----LLADSGELYRLIKAHQTA---LQDFPKRIRSYLETELGPVSAFK 5HM2 Chain:A ((7-207)) DLQKMVMANVKPVELILDGKTVALCCATGVFGTAYLVPRHLFAEKYDKVVLDGRQLDNSDFRVFEFEVKVKGQDMMSDAALMVLNRGQRVRDITMHFRDQVHIKKGTPVLGVINNADVGRLIFSGDALTYKDVVVAMDGDTMPGLFAYRAGTKVGYAGGAVMTKDGAHTVIIGTHSAGGNGVGYCSCVSRSSLLQLKAHID---

General information: TITO was launched using: RESULT: Template: 5HM2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 523 -5501 -10.52 -39.01 target 2D structure prediction score : 0.26 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -10.52 2D Compatibility (Sec. Struct. Predict.) : 0.26 1D Compatibility (Hydrophobicity) : 0.61 QMean score : -0.033

(partial model without unconserved sides chains): PDB file : Tito_5HM2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HM2-query.scw PDB file : Tito_Scwrl_5HM2.pdb: