Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKKDEHLRKPEWLKIKLNTNENYTGLKKLMRENNLHTVCEEAKCPNIHECWAVRRTATFMILGSVCTRACRFCAVKTGLP---TELDLQEPERVADSVALM-NLKHAVITAVARDDQKDGGA-GIFAETVRAIRRKSPFTTI---EVLPSDM---GGNYDNLKTLMDTRPDILNHNIETVRRLTPRVRARATYDRSLEFLRRAKEMQPDIPTKSSIMI--GLGETKEEIIEVMDDLLANNVDIMAIGQYLQPTKKHLKVQKYYHPDEFAELKEIAMQKGFSHCEAGPLVRSSYHADEQVNEASKKRQAQA 2A5H Chain:A ((121-292)) --------------------------------------------------------------ITDMCSMYCRHCTRRRFAGQSDDSMPMERIDKAIDYIRNTPQVRDVLLSGGD-ALL---VSDETLEYIIAKLREIP-HVEIVRIGSRTPVVLPQRITPELVNMLKKYHPVWLNTHFNHPNEI---------TEESTRACQLLADA--GVPLGNQSVLLRGVNDCVHVMKELVNKLVKIRVRPYYIYQC-------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2A5H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 692 -15621 -22.57 -98.24 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -22.57 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.347

(partial model without unconserved sides chains): PDB file : Tito_2A5H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2A5H-query.scw PDB file : Tito_Scwrl_2A5H.pdb: