Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVITRDFFLFLSKSGFLNKMARNWGSRIAAGKIIGGNDFNSSIPTIRQLNSQGLSVTVDHLGEFVNSAEVARERTEECIQTIATIADQELNSHVSLKMTSLGLDID---MDLVYENMTKILQTAEKHKIMVTIDMEDEVRCQKTLDIFKDFRKKY--EHVSTVLQAYLYRTEKDIDDLDSLNPFLRLVKGAYKESEKVAFPEKSDVDENYKKIIRKQLLNGHYTAIATHDDKMIDFTKQLAKEHGIANDKFEFQMLYGMRSQTQLSLVKEGYNMRVFLPYGEDWYGYFMRRLAERPSNIAFAFKGMTKK 4H6Q Chain:A ((19-299)) --------------PQVEQLARQK-MWNLAERFVAGESIESAIQAVQALERDGIAGNLDLLGEFIDSPAKCTEFADDVIKLIEAAHAAGIKPYVSIKLSSVGQGKDENGEDLGLTNARRIIAKAKEYGGFICLDMEDHTRVDVTLEQFRTLVGEFGAEHVGTVLQSYLYRSLGDRASLDDLRPNIRMVKGAYLEPATVAYPDKADVDQNYRRLVFQHLKAGNYTNVATHDERIIDDVKRFVLAHGIGKDAFEFQMLYGIRRDLQKQLAAEGYRVRVYLPYGRDWYAYFSRRIAETP-------------

General information: TITO was launched using: RESULT: Template: 4H6Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1395 3548 2.54 12.85 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 2.54 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.528

(partial model without unconserved sides chains): PDB file : Tito_4H6Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4H6Q-query.scw PDB file : Tito_Scwrl_4H6Q.pdb: