Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------------------------------------------------------------------------------------------------MNQQNQKISNPQTPVPTTSEMNDRDFVNELLT---TEKYMTTAYCTALHEFSHESLYQDIQSIFDESQKAQRKLYDLMFQYGWYSVEAEDSQKLQQSYQKFQQTIQQQSPYQQ--- 1K1A Chain:A ((125-352)) EDGDTPLHIAVVQGNLPAVHRLVNLFQQGGRELDIYNNLRQTPLHLAVITTLPSVVRLLVTAGASPMALDRHGQTAAHLACEHRSPTCLRALLDSAAPGTLDLEARNYDGLTALHVAVNTECQETVQLLLERGADIDAVDIKSGRSPLIHAVENNSLSMVQLLLQHGANVNAQMYSGSSALHSASGRG---------------LLPLVRTLVRSGA------DSSLKNCHNDTPLMVARSRRVIDILRG

General information: TITO was launched using: RESULT: Template: 1K1A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 384 27379 71.30 307.62 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : 71.30 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.150

(partial model without unconserved sides chains): PDB file : Tito_1K1A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1K1A-query.scw PDB file : Tito_Scwrl_1K1A.pdb: