Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------MSKRRMKYHSNNEISYYNFLHSMKDKIVTVYRGGPESKKGKLTAVKSDYIALQAEKKIIYYQ------LEHVKSITEDTNNSTTTIE---TEEMLDADDFHSLIGHLINQSVQFNQGGPESKKGRLVWLGDDYAALNTNED---GVVYFNIHHIKSISKHEPDLKIE-EQTPVGVLE-ADDLSEVFKSLTHKWVSINRGGP-EAIEG-ILVDNADGHY--TIVKNQEVLRIYPFHIKSISLGPKGSYKKEDQKNEQ------NQEDNNDKDSNSFISSKSYSSSKSSKRS-------------LKSSDDQSSKSGRSSRSKSSSKSSKRSLKSSDYQSSKSGRSSRSK-----------SSSKSSKRSLKSSDYQSSKSSKRSPRSSDYQSSRSPGYSSSIKSSGKQKEDYSYETIVRTIDYHWKRKF 4EPQ Chain:A ((34-472)) DYQFPLPQKNSELWIIQKKTLQDLSSGKQKLDS-FQSLES-ILEILRDSKNQNDEKYFNLKAVFEQLDKEEQTYFLEQFIPKICQLVLKIKKKQLKNQIPKESKIYEAAFSREEISYYVSCMF-LCILKDQDRKIYKDFRLIYLKDLVQQINIRRQEKIKCFYEYLKQALDFSEKESKEVVIFQRINCGQLEDYENWVDKLKAIKLKNVQLTDDKLIEDFPGTLQVDFANCDIGGGILGNGLVQEEIRFCVCPEMLVSLLVFDQSMEANEVIIMKGIKQYSDYQGYSNSFRFVKMGNSKIQKQKRTNPQTILAIDALCFNSSDNQFSEVNVSRELNKSYMGFKQEDQLKTISTGKWGCGAFLGVFDLKFAIQWIASSRSNKKMIICT-FQDEQTTKQIQQVFDLYKQKNASIFLKLVMDYPNSKYMEDYTLLEYLIELGKEKA

General information: TITO was launched using: RESULT: Template: 4EPQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1916 180671 94.30 480.51 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 94.30 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.054

(partial model without unconserved sides chains): PDB file : Tito_4EPQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EPQ-query.scw PDB file : Tito_Scwrl_4EPQ.pdb: