Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFARDIGIDLGTANVL-IHVK-GK-GIV------LNEPSVVALDKNSGKVLAVGEEARRMVGRTPGNIVAI--------------------RPLK-----DGVI---A------DFEVTEAMLKHFINKLNVK---GLFSKP-RMLICCPTNITSVEQKAIKEAAEKSGGKHVYLEEEPKVAAIGAGMEIF-------QPSGNMVVDIGGGTTDIAVISM--GDI--VT-SSSIKMAGDKFDMEILNYIKREYKLLIG-------------ERTAEDIKIKVATVFPDARHEEISIRGRDMVSGLPRTITVNSKEVEEALRESVAVIVQAAKQVLERTPPELSADIIDRGVIITGGGALLNGLDQLLAEELKVPVLVAENPMDCVAIGTGVMLDNMDKLPKRKLS 3D2F Chain:A ((2-390)) --STPFGLDLGNNNSVLAVARNRGIDIVVNEVSNRSTPSVVGFGP---KNRYLGETGKNKQTSNIKNTVANLKRIIGLDYHHPDFEQESKHFTSKLVELDDKKTGAEVRFAGEKHVFSATQLAAMFIDKVKDTVKQDTKANITDVCIAVPPWYTEEQRYNIADAARIAGLNPVRIVNDVTAAGVSYGIFKTDLPEGEEKPRIVAFVDIGHSSYTCSIMAFKKGQLKVLGTACDKHFGGRDFDLAITEHFADEFKTKYKIDIRENPKAYNRILTAAEKLKKVLSANTNA------PFSVESVMNDVDVSSQLSREELEELVKPLLERVTEPVTKALAQAKLS--A-EEVDFVEIIGGTTRIPTLKQSISEAFGKPLSTTLNQDEAIAKGAAFICAIHS--------

General information: TITO was launched using: RESULT: Template: 3D2F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1585 31534 19.89 101.39 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 19.89 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.503

(partial model without unconserved sides chains): PDB file : Tito_3D2F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3D2F-query.scw PDB file : Tito_Scwrl_3D2F.pdb: