Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGRLENKTAVITGAATGIGQATAEVFANEGARVIIGDINKDQMEETVDAIRKNGGQAESFHLDVSDENSVKAFADQIKDACGTIDILFNNAGVDQEGGKVHEYPVDLFDRIIAVDLRGTFLCSKYLIPLMLENG-GSIINTSSMSGRAADLDRSGYNAAKGGITNLTKAMAIDYARNG--IRVNSISPGTIETPLIDKLAGTKEQEMGEQFREANKWITPLGRLGQPKEMATVALFLASDDSSYVTGEDITADGGIMAYTWPGKMLIEEKWKEETK 4NBT Chain:C ((2-238)) -KKLEGKVAVITGGAKGLGQAIALAYAEEGAKVIAGDLGD---------LTYSHPNVEGMYLNVTDVTGVEKFYQSVIDKYGKIDILVNNAGITKDA-MTRKMTEAQWDAVIDVNLKGVFNLTRLVGPQMQTNGYGSIINISSVVGVFGNIGQANYAATKAGVIGLTMTWAKEFALKGANVRVNAIAPGYIMTDILK----TVPQDLLDKFAA----LTMLNRLGQPEEIAKVALFLASDDASYVTGQTINVNGGM--------------------

General information: TITO was launched using: RESULT: Template: 4NBT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1327 -66700 -50.26 -285.04 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain C : 0.78 3D Compatibility (PKB) : -50.26 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.523

(partial model without unconserved sides chains): PDB file : Tito_4NBT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NBT-query.scw PDB file : Tito_Scwrl_4NBT.pdb: