Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNINIVTIGKLKEKYLKQGIEEYTKRLSAYAKIDIIELPDEKAPENLSDQDMKIIKDKEGDRILSKISPDAHVIALAIEGKMKTSEELADTIDKLATYGKSKVTFVIGG-SLGLSDTV-MKRADEKLSFSKMTFPHQLMRLILVEQIYRAFRINRGEPYHK 3OII Chain:A ((171-252)) -----------------------------------------------------------------HLPTKCRKVTLSFDAPVIRVQDYIEKLD-----DDESICVFVGAMARGKD-NFADEYVDEKVGLSNYPLSASVACSKFCHGAEDAWNI--------

General information: TITO was launched using: RESULT: Template: 3OII.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 290 -9524 -32.84 -119.05 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -32.84 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.554

(partial model without unconserved sides chains): PDB file : Tito_3OII.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OII-query.scw PDB file : Tito_Scwrl_3OII.pdb: