Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSRKINKSRLALAITAGTLGALAQQAVAAGFIEDSKASLTLRNFYINTDNRNGTASPSKQEEWGQGFILNYQSGFTQGTVGFGVDALGLLGVRLDGGGRAGKSGLDRQPGTVFPLESNGEPVHDFASLGLTAKAKVSNTEFRYGTLQPKLPVVTYNDGRLLPVTFEGGQVTSTDLKDFTLVAGQLEHSKGRNSTDNRSLSIAGANGSSASSRDSNKFYYAGGDYKVNKDLTLQ-YYYGNLDDFYKQHFLGLIHNWQIGPGVLKTDLRAFDSSSDGKNGSRSGRADGYVSSGYYGSGVTKGEVDNRAFSGLFTYTVSGHSIGAGYQILNGDSDFPFLNRGDGEGSTAYLITDVQIGKFQRAGERTWQVRYGYDFATVGVPGLTFNTIYLSGDKIKTA--RGDQSEWERDISLAYVIPDGTFKGLGFTWKNASFRSGLPAAGSSNNQRDQDENRLIVSYTLPLL 4FMS Chain:A ((8-397)) ------------------------------GFLEDAKASLTARNFHLHR-NFVG-----KAEEWTQSFILDARSGFTQGSVGFGLDVLGLYSLKLD--------------------------ADDFGRLAVAGKLRVSNSELKIGEWMPVLPILRSDDGRSLPQTFRGGQLSANEIAGLTLYAGQFRGNSPRNDASMQDMSLFGRPAAT-----SDRFDFAGGEYRFNGERSLLGLWNAELKDIYRQQYLQLQHSQPLGDWLLGANLGGFRGRDAG-----SARA---------------GKLDNRTVSALFSARYGLHTLYLGLQKVSGDDGWMRVNGTSG----GTLANDSYNASYDNPGERSWQLRYDFDFVGLGLPGLTFMTRYLHGDHVRLAGVTDDGSEWGRESELGYTLQSGAFKRLNVRWRNSSQRRDW---------R-FDENRLIVSYPLSLL

General information: TITO was launched using: RESULT: Template: 4FMS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -1781 for 2849 contacts (-0.6/contact) + 2D Compatibility (PS) -37378 + (NN) -6269 + (LL) 2156 1D Compatibility (HY) -29200 + (ID) 7850 Total energy: -80322.0 ( -28.19 by residue) QMean score : 0.292

(partial model without unconserved sides chains): PDB file : Tito_4FMS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FMS-query.scw PDB file : Tito_Scwrl_4FMS.pdb: