Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNFVIDQNLKELTEHRTVELPVACYKTTINQNINGYIPLHWHDEIQFVLILKGIALFQINEEKIEVHEGDGLFINSGYLHMAEEKEGSDCTYICLNVSPHFVLSQELYSSYVQPYMFSTNLPYLFLAGNQQWAKNILDAVKKINQLIQQKTSLYEIDITMQLTLMWKNLVVNGFQLEYDQSEMIKSHRMKQMLNWIHLHYVEKITLEDIAKAGQLSRSECCRYFKRMLNKTPLRYVMDYRIQKSLLLLQHPESNVTEVSYQVGFNSTSYFISKFQQAMNMTPLSYKKMNS 4FE7 Chain:A ((302-407)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVN-

General information: TITO was launched using: RESULT: Template: 4FE7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 392 -62723 -160.01 -591.73 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -160.01 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.484

(partial model without unconserved sides chains): PDB file : Tito_4FE7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FE7-query.scw PDB file : Tito_Scwrl_4FE7.pdb: