Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRNRKLGIDYSSDDFEGCPVETTLDIIGGKWKGILLYHLIDGKKRFNEFRKLYPKITQRMLTLQLRELERDGVIHREVYKQVPPKVEYSLTEFGRTLEPVILHMKDWGEKYKDRIDKLEAARKAEDKI 4A5N Chain:C ((14-114)) -----------------SPVEFTLDVIGGKWKGILFYHMIDGKKRFNEFRRICPSITQRMLTLQLRELEADGIVHREVYHQVPPKVEYSLTEFGRTLEPIVLQMKEWGESNRDVLESY----------

General information: TITO was launched using: RESULT: Template: 4A5N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 284 -50263 -176.98 -497.65 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain C : 0.89 3D Compatibility (PKB) : -176.98 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.526

(partial model without unconserved sides chains): PDB file : Tito_4A5N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4A5N-query.scw PDB file : Tito_Scwrl_4A5N.pdb: