Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKKWQWLVYGWKLIILTLLTAAVFSYAMFQGGFVSWFLFYAFLPFVLYAGLLALYPLRSFQASRQMDKTQLHAGDRLGVTVTLRRKLPFPLMYMVIEDCLPEGIESLNRDGAAAKRLVFPWFKRSMTMSYELARVPRGEHHFHSVRVRTGDVLGLIEKTAFFELDDTLFVYPFYQRFSYQVNERHQEDGVSGSSPIHQHHSSVAASVRNYQPGDRFAALDWKTSARRSQLMTKEFEPSRSKNLFLLMDRFSSDAFEEVVSVTASILHSVLKNGAGAGLASIGKEKNIFPIQEGDQHFKHMLRHLAIAHCDA-ADPISRYAREELGKPSVRQADQVVVTGQLTEDML-HLAEIGG-GRVTVILAKEKDAELSQAENVMIERMMKRQIRVRIMRGGRVSRVV 2L82 Chain:A ((16-143)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KEIVREIKRQGVRVVLLYSDQDEKR-----RRERLEEFEKQGVDVRTVEDKEDFRENIREIWERYPQLDVVV-IVTTD-DKEWIKDFIEEAKERGVEVFVVYNNKD--DDRRKEAQQEFRSDGVDVRTVSDKEELIE-

General information: TITO was launched using: RESULT: Template: 2L82.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 465 -44800 -96.34 -358.40 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : -96.34 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.324

(partial model without unconserved sides chains): PDB file : Tito_2L82.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2L82-query.scw PDB file : Tito_Scwrl_2L82.pdb: