Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MTVRKEL--------IKQINLTITVIKTINQKNP--------TPMVKNILKRYEEAKEF------ILQSSDGKFEE-----DLSKVRNKLDTLTRAYL---ESANDYMNPMLKEMHKTEKLLKEYDETTQS-- 2XZ8 Chain:A ((7-141)) VKIVTRPYWLAQPPIVPLTPLKLPIESVRFVATNTPSCFTQAECTFRVRLLQNWHIESNGYKDINYNFVAAGDENIYEARGWDHSCEPPKDADELVVAFIGPSSSNKKIALELIKQGIKLGHISKNYSLIDDLEK

General information: TITO was launched using: RESULT: Template: 2XZ8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 376 -37111 -98.70 -367.43 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -98.70 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.430

(partial model without unconserved sides chains): PDB file : Tito_2XZ8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2XZ8-query.scw PDB file : Tito_Scwrl_2XZ8.pdb: