Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLYEIMLEHFAPKGSERGI---------------------FTYLLAQSDEEVYEWLKTDPSL--SDGRAVYTPYQDNEANGKTYAIYNQSFDI---------VGHEKYKDRMIRLKGELN----------------DEVELTDLYYGMTLV-----GWSMVKSDIPSEQIELLKDTGISIESA-- 5C50 Chain:B ((5-189)) -SDLDKFIKFFALKTVQVIVQARLGEKICTRSSSSPTGSDWFNLAIKDIPEVTHEAKKALAGQLPAVGRSMCVEISLKTSEGDSMELEIWCLEMNEKCDKEIKVSYTVYNRLSLLLKSLLAITRVTPAYRLSRKQGHEYVILYRIYFGEVQLSGLGEGFQTVRVGTVGTPVGTITLSCAYRINLAF

General information: TITO was launched using: RESULT: Template: 5C50.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 384 -4835 -12.59 -37.19 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -12.59 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.127

(partial model without unconserved sides chains): PDB file : Tito_5C50.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5C50-query.scw PDB file : Tito_Scwrl_5C50.pdb: