Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFSPQQPMRNYPQPGPPRPMPNQRMMGRRPPNMRGPSFGAQQQGFQQAGQFLPQANAGARNGAGAGGGLKGMLSRFLPGGGGAGSAGVPGIPGAGAAASGGAGLQGI-QNIANPASLSSMLGNVQKVLGMA-QQVTPMIQQYGPLVRNLPAMMKLYSQLSKSDDT-------------------ETEANEESEKQSVSEESSEKEKETETKTSDGNKKSKPKSSSLPKKSKTTDNKEQELKTSAPKKKEAASPAPKRTSGSSKPRLYI------------------------------------------------------- 2P03 Chain:A ((1-323)) YSREKNQPKPSPKRESGEEFRMEKLNQLWEKAQRLHLPPVRLAELHADLKIQERDELAWKKLKLDGLDEDGEKEARLIRNLNVILAKYGLDGKKDARQVTSNSLSGTQEDGLDDPRLEKLWHKAKTSGKFSGEELDKLWREFLHHKEKVHEYNVLLETLSRTEEIHENVISPSDLSDIKGSVLHSRHTELKEKLRSINQGLDRLRRVSHQGYSTEAEFEEPRVIDLWDLAQSANLTDKELEAFREELKHFEAKIEKHNHYQKQLEIAHEKLRHAESVGDGERVSRSREKHALLEGRTKELGYTVKKHLQDLSGRISRARHNEL

General information: TITO was launched using: RESULT: Template: 2P03.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 523 52595 100.56 212.93 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 100.56 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.234

(partial model without unconserved sides chains): PDB file : Tito_2P03.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P03-query.scw PDB file : Tito_Scwrl_2P03.pdb: