Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQSQKPWLAEYPNDIPHELPLPNKT-LQSILTDSAARFPDKTAISFY---------GKKLTFHDILTDALKLAAFLQCNGLQKGDRVAVMLPNCPQTVISYYGVLFAGGIVV-QTNPLYTEHELEYQLRDAQVSVIITLDLLFPKAIKMKTLSIVDQILITSVKDYLPFPKNILYPLTQKQKVHIDFDKTANIHTFASCMKQEKTELLTIPKIDPEHDIAVLQYTGGTTGAPKGVMLTHQNILANTEMCAAWMYDVKEGAEKVLGI--VPFFHVYGLTAVMNYSIKLGFEMILL-PKFDPLETLKIIDK--HKP----TLFPGAPTIYIGLLHH-----PELQHYDLSSIKSCLSGSAALPVEVKQKFEKVTGGK------LVEGYGLSEAS------------PVTHA--------NFIWGKNKPG------SIGCPWPSTDAAIYSEETGELAAPYEHGEIIVKGPQVMKGYWNKPEETAAVLRD------------------GWLFTGDMGYMDEEGFFYIADRKKDIIIAGGYNIYPREVEEALYEH-EAIQ--EIVVAGVPDSYRGETVKA-----------------FVVLKKGAKADTEELDAFARSRLAPYKVPKAYEFRK-------ELPKTAVGKILRRRLLEEETEN----HHIK 5EY9 Chain:A ((3-626)) --------YHNPFIVNGKIRFPENTNLVRHVEKWARVRGDKLAYRFLDFSTERDGVERDILWSEFSARNRAVGARLQQVT-QPGDRIAILCPQNLDYLISFFGALYSGRIAVPLFDPAEHVGRLHAVLDDCTPSTILT-----------------------TTDSAEGVRKFIRSRSAKERPRVIAVDAV-------------PTEVASTWQQPNELTTAYLQYT-----VPSGVQITHLNLPTNVLQVLNAL----EGQEGDRGVSWLPFFHDMGLITVLLASVL-GHSFTFMTPAAFVRRPGRWIRELARKPGETGGTFSAAPNFAFEHAAMRGVPRDDEPPLDLSNVKGILNGSEPVSPASMRKFFKAFEPYGLRETAVKPSYGLAEATLFVSTTPMDEVPTVIHVDRDELNKQRFVEVAADAPNAVAQVSAGKVGVDEWAVIVDTETASELPDGQIGEIWLHGNNLGIGYWGKEEESAQTFRNILKSRVPESHAEGAPDDGLWVRTGDYGTYFK-GHLYIAGRIKDLVIIDGRNHYPQDLEYTAQESTKALRVGYVAAFSVPANQLPQKVFDDPHAGLSFDPEDTSEQLVIVGERAAGTHKLEYQPIADDIRAAIAVGHGVTVRDVLLVSAGTIPRTSSGKIGRRACRTAYIDGSLRSTVFA

General information: TITO was launched using: RESULT: Template: 5EY9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2986 777 0.26 1.54 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 0.26 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.409

(partial model without unconserved sides chains): PDB file : Tito_5EY9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EY9-query.scw PDB file : Tito_Scwrl_5EY9.pdb: