Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNYIKAGKWLTVFLTFLGILLFIDLFPKEEHDQKTKSKQKPDYRAAYHFTTPDKWKNDPQKPIYFDGKYHYFYLYNRDYPKGNGTEWRHAVSEDLVHWTDEGVAIPKYTNPDGDIWTGSVVVDKENTAGFGKNALVAIVTQPSAKDKK-QEQYLWYSTDKGKSFKFYSGNPVMPNPGTDD--------------FRDPKVIWDDQDNKWVMVMAEGSKIGFYESDNLKDWHYTSGF-FPEQA-GMVECPDLYMMRASDGTNKWVLGASANGKPWGKPNTYAYWTGSFDGKEFKADQTEAQWLDYGFDWYGGVTFEDSKSTDPLEKRYALAWMNNWDY-ANNTPT-MKNGFNGTDSVIRELRLKEQDGT-YSLVSQPIEALEQLTVSTDEIEDQDVNGSKTLSITGDTYQLDTDLSWSELKNAGVRLRESEDQKRHIDVGIFAEGGYAYVNRAATNQPDKS-NTYVESKAPYDVNKRKVHLKILVDKTTIEVFVGDGKTVFSNEVFPKPEDKGITLYSDGGTASFKNITVKHFDSIHE 4FFI Chain:B ((2-478)) --------------------------------------------AVYHMTPPSGWLCNPQRPVTTHGAYQLYYLHS-DQNNGPG-GWDHASTTDGVAFTHHGTVMP--LRPDFPVWSGSAVVDTANTAGFGAGAVVALATQPTDGVRKYQEQYLYWSTDGGFTFTALP-DPVIVN--TDGRAATTPAEIENAEWFRDPKIHWDTARGEWVCVIGRLRYAAFYTSPNLRDWTLRRNFDYPNHALGGIECPDLFEITADDGTRHWVLAASMDAYGIGLPMTYAYWTGTWDGEQFHADDLTPQWLDWGWDWYAAVTWPSIDA--PETKRLAIAWMNNWKYAARDVPTDASDGYNGQNSIVRELRLARQPGGWYTLLSTPVAALTNYVTATTTLPDRTVDGSAVLPWNGRAYEIELDIAWDTATNVGISVGRSPDGTRHTNIGKY--GADLYVDRGPSDLAGYSLAPYSRAAAPIDPGARSVHLRILVDTQSVEVFVNAGHTVLSQQVHFAEGDTGISLYTDGGPAHFTGIVVREI-----

General information: TITO was launched using: RESULT: Template: 4FFI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2708 58227 21.50 127.69 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : 21.50 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.503

(partial model without unconserved sides chains): PDB file : Tito_4FFI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FFI-query.scw PDB file : Tito_Scwrl_4FFI.pdb: