Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKELSFHEKLLKLTKQQKKKTNKHVFIAIPIVFVLMFAFMWAGKAETPKVKTYSDDVLSASFVGDIMMGRYVEKVTEQKGADSIFQYVEPIFRASDYVAGNFENPVTYQKNYKQADKEIHLQTNKESVKVLKDMNFTVLNSANNHAMDYGVQGMKDTLGEFAKQNLDIVGAGYSLSDA----KKKISYQK-----VN----GVTIATLGFTDVSGKGFAAKK-NTPGVLPAD-PEIFIPMISEAK-KHADIVVVQSHWGQEYDNDPN--DRQRQLARAMSDAGADIIVGHHPHVLEPIEVYNGTVIFYSLGNFVFDQGWTRTRDSALVQYHLKKNGTGRFEVTPIDIHEATPAPVKKDSLKQKTIIRELTKDSNFAWKVEDGKLTFDIDHSDKLKSK 5EQV Chain:A ((84-253)) -----------------------------------------------------------------------------------------------------------------------------HPVYKAMNTLDYAVGNIG-NHEFNYGLDYLKKS---LAGAKFPYVNANVIDVKTGKPLFQPYLIIDTPVKDRDGKSHNLRIGYIGFVPPQVM-IWDKANLSGKVTVNDITETAKKWVPEMREQGADLVVAIPHSGLSSDPYKTMAENSVYYLSQ--VPGIDAIMFGHAHGVFPSKDF------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5EQV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 707 22586 31.95 148.59 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 31.95 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.422

(partial model without unconserved sides chains): PDB file : Tito_5EQV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EQV-query.scw PDB file : Tito_Scwrl_5EQV.pdb: