Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALDVKSRAKRYEKLDFLGEGQFATVYKARDKNTNQIVAIKKIKLGHRSEAKDGINRTALREIKLLQELSHPNIIGLLDAFGHKSNISLVFDFMETDLEVIIKDNSLVLTP-SHIKAYMLMTLQGLEYLHQHWILHRDLKPNNLLLDENGVLKLADFGLAKSFGSPNRAYTHQVVTRWYRAPELLFGARMYGVGVDMWAVGCILAELLLRVPFLPGDSDLDQLTRIFETLGTPTEEQWPDMCSLPDYV-TFKSFPGIPLHHIFSAAGDDLLDLIQGLFLFNPCARITATQALKMKYFSNRPGPTPGCQLPRPNCPVETLKEQSNPALAIKRKRTEALEQGGLPKKLIF 2WIP Chain:C ((7-301)) ---------ENFQKVEKIG-----VVYKARNKLTGEVVALKKIRLDTETE---GVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHL-----------------------------------------

General information: TITO was launched using: RESULT: Template: 2WIP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -188459 for 2299 contacts (-82.0/contact) + 2D Compatibility (PS) -30545 + (NN) -13920 + (LL) 3928 1D Compatibility (HY) -30800 + (ID) 6550 Total energy: -266346.0 ( -115.85 by residue) QMean score : 0.651

(partial model without unconserved sides chains): PDB file : Tito_2WIP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WIP-query.scw PDB file : Tito_Scwrl_2WIP.pdb: