Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHWADDDQDTSDLAYEASVKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGKVASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDGAGAVIIGEVSEGRGIISYEMGSDGTGGKHL----------YLDKDTGKLKMN--GREVFKFAVRIMGDASTRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPLSIDQELKNGKLKDDDTIVLVGFGGGLTWGAMTIKWGK 4X0O Chain:F ((8-326)) ----ITGWGKCLPPATLSNHDLSTFLDTSDEWIQSRTGIEQRRISH--VNTSDLATVAAQHAIACAGVSVEEIDLIIVATCSPDSLIPNIASRVQQNLGIPSAAAFD-LNAATGFLYGLETATRLMQASHYRHALVIGAERLSFYLDWTKRDTAVLFGDGAGAVVLSKTEQKVGLQDAQIGCDAQGRDILAVPKFGTAMDRFDADNGYWAFDFVGKEIFKRAVRGMGAAAQQVLARSGLSTEEIDVVIPHQANIRIIQTLCDLAGIAQDKAFVNIHRYGNTSAATVPIALCEALEQGKIKPHDDLLVAAFGAGLTWGAGHIRWGE

General information: TITO was launched using: RESULT: Template: 4X0O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -177226 for 2822 contacts (-62.8/contact) + 2D Compatibility (PS) -33206 + (NN) -17211 + (LL) 116 1D Compatibility (HY) -24000 + (ID) 6750 Total energy: -258277.0 ( -91.52 by residue) QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_4X0O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4X0O-query.scw PDB file : Tito_Scwrl_4X0O.pdb: