Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAGVLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDIDRLLENFKGVIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDFKENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSL-TLEQI-------KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVFIDDDSEKVLAIYMVHPTKESEIKPKKVFN 2RFK Chain:A ((43-256)) ---GVINLDKPPGPTSHEVVAWIKKILNLEKAGHGGTLAPKVSGVLPVALEKATRVVQALLPAGKEYVALMHL--------------------HGDVPEDKIIQVMKEFEGEIIQRPPLRSAV--------------------KRRLRTRKVYYIEVLEIEGRDVLFRVGVEA--GTYIRSLIHHIGLALGVGAHMSELRRTRSGPFKEDETLITLHDLVDYYYFWKEDGIEEYFRKAIQPMEKAVEHLPKVWIKDSAV------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2RFK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -125230 for 1442 contacts (-86.8/contact) + 2D Compatibility (PS) -21607 + (NN) -3828 + (LL) 5308 1D Compatibility (HY) -15600 + (ID) 4050 Total energy: -165007.0 ( -114.43 by residue) QMean score : 0.430

(partial model without unconserved sides chains): PDB file : Tito_2RFK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RFK-query.scw PDB file : Tito_Scwrl_2RFK.pdb: