Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTIQDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQEKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQTVKVTGGEEQLNDIAYLKATFKTNKKINGDTKDVAEVTAFDKKLNKLNVSIQPNEVNLQVKVEPFSKKVKVNVKQKGSLADDKELSSI-----DLEDKEIEIFGSRDDLQNISEVDAEVDLDGISESTEKTVKINLPEHVTKAQ-PSETKAYINVK----- 4LFU Chain:A ((5-240)) -DFFSWRRTMLLRFQRM------------------------ETAEEVYHEIELQAQQLEYDYYSLCVRHPVPFTRPKVAFYTNYP-------------EAWVSYYQAKNFLA---------------------------------IDPVLNPENFSQGHLMW----NDDLFSEAQPLWEAARAH----GLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRESLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSI------SENTVNFHQKNMQKKINAPNKTQVACYAAATGLI

General information: TITO was launched using: RESULT: Template: 4LFU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) 32611 for 1624 contacts (20.1/contact) + 2D Compatibility (PS) -23538 + (NN) -4460 + (LL) 4652 1D Compatibility (HY) -5600 + (ID) 1500 Total energy: 2165.0 ( 1.33 by residue) QMean score : 0.164

(partial model without unconserved sides chains): PDB file : Tito_4LFU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LFU-query.scw PDB file : Tito_Scwrl_4LFU.pdb: