Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MGGLQTTGYAENASF---SQLSACFSSRLVREDLFLMFSA---YNQFTLLHSRLTMISYNGDDQ 4F3F Chain:C ((6-64)) MCPSGKKAREIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKHKLDEL-------

General information: TITO was launched using: RESULT: Template: 4F3F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 131 -22610 -172.60 -443.33 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain C : 0.70 3D Compatibility (PKB) : -172.60 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_4F3F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4F3F-query.scw PDB file : Tito_Scwrl_4F3F.pdb: