Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTSIEQKPFNRSVIVGILLAGAFVAILNQTLLITALP-HIMRDFNVDANQAQWLTTSFMLTNGILIPITAFLIEKF-TSRALLITAMSIFTAGTVVGAFAP-NFPVLLTARIIQAAGAGIMMPLMQTVFLTIFPIE-KRGQAMGMVGLVISFAPAIGPTLSGWAVEA--------FSWRSLFYIILPFAVIDLILASILMKNVTTLRKTQIDILSVILSTF-------GFGGLLYGFSSVGSYGWSSSTVLISLLVGVIALLLFITRQMKLKKPMLEFRVFTFGVFSLTTLLGTLVFALLIGTETILPLYTQNVRDVT---------AFDTGLMLLPGAVVMGFMSPIIGRIFDRVGG-RGLAIAGFCIIFLTSLPFMQLTD-----HTSLAWIVVLYTVRLLGTAMIMMPVTTAGINALPRHLIPHGTAMNNTIRQVGGSIGTALLVSVMSNQAAHAGTTNVKHAALHGMNAAFIVAAVIALVGFLLSFTLKKPQRPAEQQPAR 4TPH Chain:A ((17-449)) ------------------VSLIELWERFGYYGMQALIVYFMVQRLGFDDSRANLVWSACAALIYVSPAIGGWVGDKILGTKRTMLLGAGILSVGYALMTVPTENTWFMFSALGVIVVGNGLFKPNAGNLVRKIYE--SKIDSAFTIYYMAVNVGSTFSMLLTPWIKDYVNAQYGNEFGWHAAFAVCCVGILVGLGNYA-LMHKSLANYGSEPDTRPVNKKSLAIVLALAALSVVASAIIL-EYEDVARV---FVYAAGVAVLGIFFHL--------ERAGLIAAL---ILTVQTVFFFIFYQQMSTSLALFALRNVDWDFQVFGTHLWTWSPAQFQALNPIWIMVLSPVLAW-------IAAKFALGFAVVAIGFFIYGFAGQFAVNGKTSSWVMIWGYASYSLGELLVSGLGLAMIAR---------MMGAYFVASGISQYLGGVVANFASV-----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4TPH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1851 -281974 -152.34 -768.32 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -152.34 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.323

(partial model without unconserved sides chains): PDB file : Tito_4TPH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TPH-query.scw PDB file : Tito_Scwrl_4TPH.pdb: