Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVRYQIEQLGDSAMMIRFGEEINEQVNGIVHAAAAYIEEQPFPGFIECIPAFTSLTVFYDMYEVYKH--LPQGISSPFESVKRDVEERLAEIAEDYEVNRRIVEIPVCYGGEFGPDLEEVAKINQLSPEEVIDIHTNGEYVVYMLGFAPGFPFLGGMSKRIAAPRKSSPRPSIPAGSVGIAGLQTGVYPISTPGGWQLIGKTPLALFRPQENPPTLLRAGDIVKFVRISEKDYHAYKEESN 3MML Chain:B ((20-228)) --TLGTVHNYGDQALLLEFDS------TAEVLAWTETLREAELLGVVDIVPAARTVLVKLAGPRYQAPTRQRLG------KLRVRPEAIT--H--QPPGDRVDVTIDVVYDG---ADLHEVASLTGMTPAQVIAAHTGTPWRVGFCGFAPGFAYLVDGDARLQVPRRAEPRTSVPAGAVALAGEFSGVYPRQSPGGWQLIGHTDAVMFDVNRDKPALLTPGMWVQFRAVG------------

General information: TITO was launched using: RESULT: Template: 3MML.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1040 -155633 -149.65 -751.85 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -149.65 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_3MML.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MML-query.scw PDB file : Tito_Scwrl_3MML.pdb: