Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEISKQTSDLLLSLEKKKGTLPKFSVLRSIPRNRIIYGAPGTGKSNYLEREVGKIFGDNPYVFTRVTFFPGYTYGQFIGAYKPVPIYKKLSGEEEIFSSNFRDKMENFEPMIDYQFVPGPFIDVLIKALKNRYTNFILIIEEINRANAASVFGDIFQLLDRNKNGESDYPVTFGPDIMNYLARNGIKDEMIKLPSNFFIWATMNNADQGVLPLDTAFKR--RWSFE-YLELEKYRKAVDSWKLSLRYKGHNKVIMWNDFRDIINKRLKGKVPEDKLLGPFFLKESELWNQNVFKNKLLYYLKEDVFKHNPTIDFLNASTFSELIEKYDGSDNIFTFDIDDSSFVSD 5FTJ Chain:A ((339-405)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AHVIVMAATNRPN----SIDPALRRFGRFDREVDIGIPDATGRLEILQIHT-----------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FTJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 43 -3116 -72.47 -70.82 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : -72.47 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.289

(partial model without unconserved sides chains): PDB file : Tito_5FTJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FTJ-query.scw PDB file : Tito_Scwrl_5FTJ.pdb: