Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MAKPKKKKFEVTEQ--QTIDAVLQQMKEEGYLPVRRMEEPIFMEKKENGSIQIVPCGKKIVFEGKLI 4M7D Chain:A ((3-67)) ATLKDYLNKRVVIILVDGESLIASLNGFDKNTNLFLTNVFNRISKEFISKAQLL---RGSEIALVGLI-

General information: TITO was launched using: RESULT: Template: 4M7D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 250 -22162 -88.65 -363.31 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -88.65 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.057

(partial model without unconserved sides chains): PDB file : Tito_4M7D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M7D-query.scw PDB file : Tito_Scwrl_4M7D.pdb: